MCDB 101L - Molecular Genetics Laboratory
Current molecular genetics techniques used to study model organisms. Module1 - Forward genetics: Mutagenesis and genetic mapping of mutants using single nucleotide polymorphisms, classical Mendelian inheritance. 2) Reverse genetics: gene knockdown by RNAi. 3) Genetic analysis of cell signaling. 4) Cellular reprogramming using transgenic strains. Techniques: PCR, gel electrophoresis, Computer based sequence analysis, fluorescence and light microscopy and imaging. Experiments are based on ongoing research at UCSB. Students get to design their own experiment in final module.
Pradeep Joshi
2.0